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mbmp4  (R&D Systems)


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    R&D Systems mbmp4
    Mbmp4, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mbmp4/mbmp4/pmc05718193-448-30-33
    Average 90 stars, based on 1 article reviews
    mbmp4 - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Nodal signaling is required for mesodermal and ventral but not for dorsal fates in the indirect developing hemichordate, Ptychodera flava
    Article Snippet: The reagents used were: mNodal (#1315-ND), hNodal (#3218-ND), mLefty (#994-LF), zBmp4 (#1128-BM), mBmp4 (#5020-BP) and hNoggin (#6057-NG) from R&D Systems, Inc; SB431542 (#S4317), Dorsomorphin (#P5499) and U0126 (Sigma, #U120) from Sigma Aldrich Co, LLC.

    Article Title: Distinct mesenchymal lineages and niches promote epithelial self-renewal and myofibrogenesis in the lung
    Article Snippet: Ligand treatments of organoids were performed using the following reagents at the indicated concentrations, mIL-6 50ng/ml (R&D Systems), STATTIC 2 and 20 μM (Selleck BioChemicals), mGREM2 25 ng/ml (R&D Systems), mBMP4 50 ng/ml (R&D Systems), FGF7 25 ng/ml (R&D Systems).

    Article Title: Snai1 promotes ESC exit from the pluripotency by direct repression of self-renewal genes.
    Article Snippet: TGF‐β1 (240‐B‐010), mBMP4 (5020‐BP‐010) and Activin A (338‐AC‐010) were obtained from R&D System. bFGF (AA‐10‐155) was ob‐ tained from Life Technology.

    Article Title: Differentiation of stem cells into thyroid tissue
    Article Snippet: For Anteriorization of endoderm, on day 5 (120 total hours of differentiation) EBs were plated onto p100 Petri dishes in Nog/SB media: cSFDM supplemented with 100 ng/ml mNoggin (R&D 1967-NG) and 10 μM SB431542 (Sigma S4317) as previously described (Longmire et al., 2012).

    Expressing:

    Article Title: Regeneration of thyroid function by transplantation of differentiated pluripotent stem cells
    Article Snippet: Next, 100 ng/ml mNoggin (R&D) and 10 μM SB431542 (Sigma) was applied for 24 hours to generate anterior foregut endoderm. .. Expression of Nkx2-1 was induced with specification media containing 100 ng/ml mWnt3a, 10 ng/ml mKGF, 10 ng/ml hFGF10, 10 ng/ml mBMP4, 20 ng/ml hEGF, and 250 ng/ml mFGF2 (all from R&D). ..

    Cell Culture:

    Article Title: Hypoxia Enhances Differentiation of Mouse Embryonic Stem Cells into Definitive Endoderm and Distal Lung Cells
    Article Snippet: .. Briefly, following differentiation of the mESC to DE (as described previously), differentiation toward anterior foregut endoderm (anteriorization) was initiated by switching the culture to the SP media supplemented with 100 ng/mL mNoggin (R&D Systems) and 10 μM SB431542 (R&D Systems) for 24 h. Next, to induce lung-specification, the early lung/thyroid progenitors were cultured in the SP media supplemented with 100 ng/mL mWnt3a (R&D Systems), 10 ng/mL mouse keratinocyte growth factor (mKGF) (R&D Systems), 10 ng/mL human fibroblast growth factor 10 (hFGF10) (R&D Systems), 10 ng/mL mBMP4 (R&D Systems), 20 ng/mL human epidermal growth factor (hEGF) (R&D Systems), 500 ng/mL mouse fibroblast growth factor 2 (mFGF2) (R&D systems), and 100 ng/mL heparin sodium salt (Sigma) for 7 days. .. Thereafter, the cells were cultured for 6 days in the induction media (induction) supplemented with 100 ng/mL hFGF10, 500 ng/mL mFGF2, and 100 ng/mL heparin, followed by culture for 3 days in the distal lung differentiation media supplemented with 50 nM dexamethasone (Sigma), 0.1 mM cAMP (Sigma), 0.1 mM 3-isobutyl-1-methylxanthine (IBMX) (Sigma), and 10 ng/mL mKGF.



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    Figure 4. GNU-mKATE2 co-localizes with <t>TRAL-GFP</t> and ME31B-GFP granules in mature oocytes. Mature oocytes were isolated from gnuwt-mkate2; tral-gfp or me31b-gfp;gnuwt-mkate2 females, fixed, and the vitelline membrane removed manually. Oocytes were stained with the anti-GFP booster and imaged by confocal microscopy for <t>fluorescence</t> at 488 nm to detect GFP and 568 nm to detect mKATE2. mKATE2 signal was detected without the use of a booster. Co-localization was measured by quantification of overlap between GFP+ granules and mKATE2+ granules using the surface- surface co-localization algorithm in Imaris (Bitplane). (A) Representative image of gnuwt-mkate2;tral-gfp oocytes. Co-localizing GNU-mKATE2 (magenta) and TRAL-GFP (green) granules are colored in white. The images shown are single slices of confocal z-stacks from one oocyte. Bottom images show the insets of each panel (dashed yellow box). Scale bar represents 20 mm. (A’) Venn diagram of quantified co-localization between GNU and TRAL granules. GNU and TRAL co-localize in 33.6±5.2% of all granules quantified. GNU-containing TRAL granules represent approximately a third of TRAL granules scored. Values are averaged across eight oocytes. (B) Representative image of me31b-gfp;gnuwt-mkate2 oocytes. Co-localizing GNU-mKATE2 (magenta) and ME31B-GFP (green) granules are colored in white. The images shown are single slices of confocal z-stack from one oocyte. Bottom images show the insets of each panel (dashed yellow box). Scale bar represents 20 mm. (B’) Venn diagram of quantified co-localization between GNU and ME31B granules. GNU and ME31B co-localize in 57.8±4.6% of all granules quantified. GNU-containing ME31B granules represent approximately half of ME31B granules scored. Values are averaged across eight oocytes. The online version of this article includes the following source data and figure supplement(s) for figure 4:
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    Figure 4. GNU-mKATE2 co-localizes with <t>TRAL-GFP</t> and ME31B-GFP granules in mature oocytes. Mature oocytes were isolated from gnuwt-mkate2; tral-gfp or me31b-gfp;gnuwt-mkate2 females, fixed, and the vitelline membrane removed manually. Oocytes were stained with the anti-GFP booster and imaged by confocal microscopy for <t>fluorescence</t> at 488 nm to detect GFP and 568 nm to detect mKATE2. mKATE2 signal was detected without the use of a booster. Co-localization was measured by quantification of overlap between GFP+ granules and mKATE2+ granules using the surface- surface co-localization algorithm in Imaris (Bitplane). (A) Representative image of gnuwt-mkate2;tral-gfp oocytes. Co-localizing GNU-mKATE2 (magenta) and TRAL-GFP (green) granules are colored in white. The images shown are single slices of confocal z-stacks from one oocyte. Bottom images show the insets of each panel (dashed yellow box). Scale bar represents 20 mm. (A’) Venn diagram of quantified co-localization between GNU and TRAL granules. GNU and TRAL co-localize in 33.6±5.2% of all granules quantified. GNU-containing TRAL granules represent approximately a third of TRAL granules scored. Values are averaged across eight oocytes. (B) Representative image of me31b-gfp;gnuwt-mkate2 oocytes. Co-localizing GNU-mKATE2 (magenta) and ME31B-GFP (green) granules are colored in white. The images shown are single slices of confocal z-stack from one oocyte. Bottom images show the insets of each panel (dashed yellow box). Scale bar represents 20 mm. (B’) Venn diagram of quantified co-localization between GNU and ME31B granules. GNU and ME31B co-localize in 57.8±4.6% of all granules quantified. GNU-containing ME31B granules represent approximately half of ME31B granules scored. Values are averaged across eight oocytes. The online version of this article includes the following source data and figure supplement(s) for figure 4:
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    Figure 4. GNU-mKATE2 co-localizes with <t>TRAL-GFP</t> and ME31B-GFP granules in mature oocytes. Mature oocytes were isolated from gnuwt-mkate2; tral-gfp or me31b-gfp;gnuwt-mkate2 females, fixed, and the vitelline membrane removed manually. Oocytes were stained with the anti-GFP booster and imaged by confocal microscopy for <t>fluorescence</t> at 488 nm to detect GFP and 568 nm to detect mKATE2. mKATE2 signal was detected without the use of a booster. Co-localization was measured by quantification of overlap between GFP+ granules and mKATE2+ granules using the surface- surface co-localization algorithm in Imaris (Bitplane). (A) Representative image of gnuwt-mkate2;tral-gfp oocytes. Co-localizing GNU-mKATE2 (magenta) and TRAL-GFP (green) granules are colored in white. The images shown are single slices of confocal z-stacks from one oocyte. Bottom images show the insets of each panel (dashed yellow box). Scale bar represents 20 mm. (A’) Venn diagram of quantified co-localization between GNU and TRAL granules. GNU and TRAL co-localize in 33.6±5.2% of all granules quantified. GNU-containing TRAL granules represent approximately a third of TRAL granules scored. Values are averaged across eight oocytes. (B) Representative image of me31b-gfp;gnuwt-mkate2 oocytes. Co-localizing GNU-mKATE2 (magenta) and ME31B-GFP (green) granules are colored in white. The images shown are single slices of confocal z-stack from one oocyte. Bottom images show the insets of each panel (dashed yellow box). Scale bar represents 20 mm. (B’) Venn diagram of quantified co-localization between GNU and ME31B granules. GNU and ME31B co-localize in 57.8±4.6% of all granules quantified. GNU-containing ME31B granules represent approximately half of ME31B granules scored. Values are averaged across eight oocytes. The online version of this article includes the following source data and figure supplement(s) for figure 4:
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    Figure 4. GNU-mKATE2 co-localizes with <t>TRAL-GFP</t> and ME31B-GFP granules in mature oocytes. Mature oocytes were isolated from gnuwt-mkate2; tral-gfp or me31b-gfp;gnuwt-mkate2 females, fixed, and the vitelline membrane removed manually. Oocytes were stained with the anti-GFP booster and imaged by confocal microscopy for <t>fluorescence</t> at 488 nm to detect GFP and 568 nm to detect mKATE2. mKATE2 signal was detected without the use of a booster. Co-localization was measured by quantification of overlap between GFP+ granules and mKATE2+ granules using the surface- surface co-localization algorithm in Imaris (Bitplane). (A) Representative image of gnuwt-mkate2;tral-gfp oocytes. Co-localizing GNU-mKATE2 (magenta) and TRAL-GFP (green) granules are colored in white. The images shown are single slices of confocal z-stacks from one oocyte. Bottom images show the insets of each panel (dashed yellow box). Scale bar represents 20 mm. (A’) Venn diagram of quantified co-localization between GNU and TRAL granules. GNU and TRAL co-localize in 33.6±5.2% of all granules quantified. GNU-containing TRAL granules represent approximately a third of TRAL granules scored. Values are averaged across eight oocytes. (B) Representative image of me31b-gfp;gnuwt-mkate2 oocytes. Co-localizing GNU-mKATE2 (magenta) and ME31B-GFP (green) granules are colored in white. The images shown are single slices of confocal z-stack from one oocyte. Bottom images show the insets of each panel (dashed yellow box). Scale bar represents 20 mm. (B’) Venn diagram of quantified co-localization between GNU and ME31B granules. GNU and ME31B co-localize in 57.8±4.6% of all granules quantified. GNU-containing ME31B granules represent approximately half of ME31B granules scored. Values are averaged across eight oocytes. The online version of this article includes the following source data and figure supplement(s) for figure 4:
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    Figure 4. GNU-mKATE2 co-localizes with <t>TRAL-GFP</t> and ME31B-GFP granules in mature oocytes. Mature oocytes were isolated from gnuwt-mkate2; tral-gfp or me31b-gfp;gnuwt-mkate2 females, fixed, and the vitelline membrane removed manually. Oocytes were stained with the anti-GFP booster and imaged by confocal microscopy for <t>fluorescence</t> at 488 nm to detect GFP and 568 nm to detect mKATE2. mKATE2 signal was detected without the use of a booster. Co-localization was measured by quantification of overlap between GFP+ granules and mKATE2+ granules using the surface- surface co-localization algorithm in Imaris (Bitplane). (A) Representative image of gnuwt-mkate2;tral-gfp oocytes. Co-localizing GNU-mKATE2 (magenta) and TRAL-GFP (green) granules are colored in white. The images shown are single slices of confocal z-stacks from one oocyte. Bottom images show the insets of each panel (dashed yellow box). Scale bar represents 20 mm. (A’) Venn diagram of quantified co-localization between GNU and TRAL granules. GNU and TRAL co-localize in 33.6±5.2% of all granules quantified. GNU-containing TRAL granules represent approximately a third of TRAL granules scored. Values are averaged across eight oocytes. (B) Representative image of me31b-gfp;gnuwt-mkate2 oocytes. Co-localizing GNU-mKATE2 (magenta) and ME31B-GFP (green) granules are colored in white. The images shown are single slices of confocal z-stack from one oocyte. Bottom images show the insets of each panel (dashed yellow box). Scale bar represents 20 mm. (B’) Venn diagram of quantified co-localization between GNU and ME31B granules. GNU and ME31B co-localize in 57.8±4.6% of all granules quantified. GNU-containing ME31B granules represent approximately half of ME31B granules scored. Values are averaged across eight oocytes. The online version of this article includes the following source data and figure supplement(s) for figure 4:
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    Figure 4. GNU-mKATE2 co-localizes with TRAL-GFP and ME31B-GFP granules in mature oocytes. Mature oocytes were isolated from gnuwt-mkate2; tral-gfp or me31b-gfp;gnuwt-mkate2 females, fixed, and the vitelline membrane removed manually. Oocytes were stained with the anti-GFP booster and imaged by confocal microscopy for fluorescence at 488 nm to detect GFP and 568 nm to detect mKATE2. mKATE2 signal was detected without the use of a booster. Co-localization was measured by quantification of overlap between GFP+ granules and mKATE2+ granules using the surface- surface co-localization algorithm in Imaris (Bitplane). (A) Representative image of gnuwt-mkate2;tral-gfp oocytes. Co-localizing GNU-mKATE2 (magenta) and TRAL-GFP (green) granules are colored in white. The images shown are single slices of confocal z-stacks from one oocyte. Bottom images show the insets of each panel (dashed yellow box). Scale bar represents 20 mm. (A’) Venn diagram of quantified co-localization between GNU and TRAL granules. GNU and TRAL co-localize in 33.6±5.2% of all granules quantified. GNU-containing TRAL granules represent approximately a third of TRAL granules scored. Values are averaged across eight oocytes. (B) Representative image of me31b-gfp;gnuwt-mkate2 oocytes. Co-localizing GNU-mKATE2 (magenta) and ME31B-GFP (green) granules are colored in white. The images shown are single slices of confocal z-stack from one oocyte. Bottom images show the insets of each panel (dashed yellow box). Scale bar represents 20 mm. (B’) Venn diagram of quantified co-localization between GNU and ME31B granules. GNU and ME31B co-localize in 57.8±4.6% of all granules quantified. GNU-containing ME31B granules represent approximately half of ME31B granules scored. Values are averaged across eight oocytes. The online version of this article includes the following source data and figure supplement(s) for figure 4:

    Journal: eLife

    Article Title: The GNU subunit of PNG kinase, the developmental regulator of mRNA translation, binds BIC-C to localize to RNP granules

    doi: 10.7554/elife.67294

    Figure Lengend Snippet: Figure 4. GNU-mKATE2 co-localizes with TRAL-GFP and ME31B-GFP granules in mature oocytes. Mature oocytes were isolated from gnuwt-mkate2; tral-gfp or me31b-gfp;gnuwt-mkate2 females, fixed, and the vitelline membrane removed manually. Oocytes were stained with the anti-GFP booster and imaged by confocal microscopy for fluorescence at 488 nm to detect GFP and 568 nm to detect mKATE2. mKATE2 signal was detected without the use of a booster. Co-localization was measured by quantification of overlap between GFP+ granules and mKATE2+ granules using the surface- surface co-localization algorithm in Imaris (Bitplane). (A) Representative image of gnuwt-mkate2;tral-gfp oocytes. Co-localizing GNU-mKATE2 (magenta) and TRAL-GFP (green) granules are colored in white. The images shown are single slices of confocal z-stacks from one oocyte. Bottom images show the insets of each panel (dashed yellow box). Scale bar represents 20 mm. (A’) Venn diagram of quantified co-localization between GNU and TRAL granules. GNU and TRAL co-localize in 33.6±5.2% of all granules quantified. GNU-containing TRAL granules represent approximately a third of TRAL granules scored. Values are averaged across eight oocytes. (B) Representative image of me31b-gfp;gnuwt-mkate2 oocytes. Co-localizing GNU-mKATE2 (magenta) and ME31B-GFP (green) granules are colored in white. The images shown are single slices of confocal z-stack from one oocyte. Bottom images show the insets of each panel (dashed yellow box). Scale bar represents 20 mm. (B’) Venn diagram of quantified co-localization between GNU and ME31B granules. GNU and ME31B co-localize in 57.8±4.6% of all granules quantified. GNU-containing ME31B granules represent approximately half of ME31B granules scored. Values are averaged across eight oocytes. The online version of this article includes the following source data and figure supplement(s) for figure 4:

    Article Snippet: DOI: https://doi.org/10.7554/eLife.67294 20 of 27 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody Donkey polyclonal HRP-conjugated anti-rabbit IgG Jackson Immuno Research Jackson Immuno Research: 711-035-152; RRID:AB_10015282 Antibody Recombinant Nanobody gba488-100 (GFP-Booster Atto488) Chromotek Booster for GFP fluorescence (1/400) in diluent buffer Recombinant DNA reagent pCasPeR4_gnu-wt-gfp Hara et al., 2017 RRID:Addgene_113005 Recombinant DNA reagent pCasPeR4_gnu-DSAM-gfp This paper Generated from pCas PeR4_gnu-wt-gfp Recombinant DNA reagent pCasPeR4_gnu-mkate2 This paper Generated from pCas PeR4_gnu-wt-gfp Commercial assay or kit gtm_20 anti-GFP magnetic beads Chromotek GFP immunoprecipitation Chemical compound, drug HIKARI signal enhancer Nacalai Nacalai: 02270–81 Signal Enhancer HIKARI for Western Blotting and ELISA Software, algorithm Imaris (Bitplane) Bitplane Imaging data visualization and analysis Software, algorithm FIJI (ImageJ) ImageJ Imaging data visualization and analysis Software, algorithm Scaffold (version 4) Proteome Software Proteomic data visualization and analysis

    Techniques: Isolation, Membrane, Staining, Confocal Microscopy, Fluorescence